Sushil Dhungana

About Me

An enthusiast of research & services integrated career in Biotechnology

After completion of my B.tech in Biotechnology from Kathmandu University in 2019, I have been researching and employed in the field of molecular plant pathogen detection & bio-control agents. As an entrepreneur, I am also industrially producing mushroom spawns being based on GMP. Other short-term researches that I have contributed to revolve around optimization of production of mushroom, secondary foods, slow sand filtrers, and organizational management.

Personally, I wake up every day to: embrace opportunities, harness resourcefullness, & take risks in the pursuit of meaningful progress. 

'Genesis'  is the word that drives me.

  Key Interests:  Molecular Biology, Industrial / Agricultural Biotechnology, Immunology, Bio-informatics, Genomics & Proteomics
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Education
Department of Biotechnology, Kathmandu University

  Dhulikhel, Kavre, Nepal

01/08/2015 – 14/11/2019

Bachelors of Technology in the Field of Biotechnology (EQF Level: 6)

CGPA 3.71 (Equivalent to 89.85%)

White House Secondary School And College "Himalayan White House International College"

  Khumal Heights, Lalitpur, Kathmandu, Nepal

01/08/2011 – 19/08/2013

Higher Secondary Education Board (EQF Level: 5)

83.10%

Balodaya English Boarding Secondary School

  Balodaya Marga, Birauta, Pokhara-17

14/04/1998 – 20/06/2011

School Leaving Certificate (EQF Level: 4)

88.38%

Experience
Integrated Rural Development Program, Nepal Technology Innovation Center

  Dhulikhel, Kavre, Nepal

01/01/2019 – 30/11/2020

Research Assistant  

Project title: Development of River Water Pump for drinking and irrigation purposes in rural areas to provide the means of pumping water without use of electricity and fuel. 

Principal Investigator: Prof. Hari Prasad Neopane

Key Responsibilities Completed:

Literature Review, Optimization of water filtration by slow sand filters (using DOE and RSM), prototyping, water quality testing, field surveys and documentations (proposals, progress reports and log sheets).

Green Bio Tech And Research Center Pvt. Ltd

  Pokhara-14, Chauthe, Gandaki Province, Nepal

12/02/2020 – Present 

Co-Founder and Chief Technology Officer 

Key Responsibilities Completed and Undergoing:

Completed:  Planning, infrastructural design and procurement of equipment for laboratory,
Ongoing:

  • Operation of the microbiology lab and management of cultures,
  • Organize the production of company along with its Quality assessment,
  • Collaborate with various institutions for inception, proposal, management and completion of various product based research projects.
  • Private and public relation management.

Currently the production and research of this enterprise is focuesd on spawns of straw mushrooms (Pleurotus ostreatus, P. florida, P. eryngii), button mushroom (Agaricus bisporus), and shitake-wood mushroom (Lentinula edodes) as well as their cultivation trials and secondary product development based on mushroom.

Integrated Rural Development Program, Nepal Technology Innovation Center

  Syangja And Pokhara, Gandaki, Nepal

1/08/2022 – 8/09/2023

Project Coordinator  

Project title: Development of human health promoting fumigates from raw materials cultivated and available in rural areas for strengthening health security.

Principal Investigator: Dr. Bibas Bastola

Key Responsibilities:

  • To coordinate research team, research collaborators, industrial partners, surveyers, experts and other project stake-holders for timely completion of the project.
  • To facilitate communication of the findings of the project to scientific community via conferences, workshops and media.
  • To support the purchase / leasing of equipment, raw materials, research spaces, product trial spaces and essentials for viability of research for the project.
  • To support overall documentations required for project inclusive of project proposals, presentations, progress reports, log sheets, and final reports.

Shikhar Biotech Pvt. Ltd

  Kathmandu, Nepal

14/03/2019 – 03/04/2019
Biotech Intern 

As an intern my hands-on practices were regarding Goat poly-clonal antibodies isolation & validation using: ELISA, SDS-page, Western Blotting, Immunofluorescence and Flow Cytometry

Plant Protection Laboratory, Government of Nepal

  Pokhara, Gandaki Province, Nepal

18/09/2019 – 16/07/2023 

Biotechnologist 

Key Responsibilities Completed:

Molecular Laboratory: 

  • Infrastructural Design, procurements of equipments and reagents. 
  • Development of optimized protocols for PCR detection of Citrus Greening Disease in Gandaki Province, Nepal.
  • Field surveys and sample collection for disease diagnosis based on geographical distribution over the province. 
  • Analysis, documentation, and communication of findings.

Microbiology Laboratory:

  • Research and Development of bio-control agents: isolation, characterization, efficacy assays, pure culture maintenance, and field trials. 
  • Mass scale production of Trichoderma (T. viride & T. harzianum) for distribution to farmers. 
  • Plant Pathogen Detection based on samples collected. 

Projects
Publications & Conferences
Optimization of Mushroom Spawns of Pleurotus sp. for Industrial Scale Production.
Manuscript Being Written
Optimization of Production of Dried Oyster Mushroom Products using Response Surface Methodology.`
Manuscript being written
Implementation of Good Agricultural Practices in mushroom cultivation.
Manuscript being written currently
Mathematical Modelling for the Bacterial Poly-hydroxybutyrate Production using Response Surface Methodology
Manuscript Submitted to MDPI
Technical Skills
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Design of Experiments (DoE)
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Response Surface Methodology for Optimization
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Design Expert
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R (Programming Language) Intermediate User
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Google CoLaboratory and Python (Intermediate)
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Molecular Evolutionary Genetic Analysis (MEGA)
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BioEdit
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AutoCAD & SolidWorks
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LaTex (Begineer User)
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Microsoft Office Package
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WordPress
Certifications
Workshop on Metabolic Modelling
(28/06/2018 – 03/07/2018) Oxford Brookes University Research Center for Applied Science and Technology (RECAST) & SBAN, Tribhuwan University
Optimization of Polyhydroxybutyrate & Production in Bacteria by Response Surface Methodology and Production in Molasses.
(28/01/2020 – 31/01/2020) 3rd International Conference on Bioscience & Biotechnology, Research Institute for Bioscience and Biotechnology (RIBB), Nepal
International Workshop on Advancing Biological and Clinical Research with Flowcytometry – 2019
(02/03/2019 – 06/03/2019) Central Department of Biotechnology, Tribhuwan University; Ministry of Science & Technology, Government of Nepal
Awards